Definition: In the field of biochemistry, "autofluorescence" refers to a phenomenon where a molecule undergoes fluorescence without any energy input from light. This can be observed in biological systems such as cells or tissues, which have a high concentration of fluorescent molecules like pyrimidine diphosphate (PiP) or cytidine deoxyribonucleotide (cDNA). When these molecules are exposed to light, they fluoresce at different wavelengths, creating a unique color pattern. This
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