👉 Donor engineering is a sophisticated biotechnological strategy used to optimize the delivery and integration of therapeutic genes into target cells, particularly in gene therapy applications. This process involves modifying the genetic material (donor vector) to enhance its efficiency, safety, and specificity. By altering the donor vector's components, such as promoters, enhancers, and regulatory sequences, researchers can control gene expression levels, ensure targeted delivery to specific cell types, and minimize potential adverse effects like immune responses or insertional mutagenesis. Advanced techniques, including CRISPR/Cas9-mediated genome editing and synthetic biology approaches, are often employed to engineer donor vectors with precise modifications. This method is crucial for improving the efficacy and tolerability of gene therapies, making it a cornerstone in the development of novel treatments for genetic disorders and other diseases.