Definition: Spectrophotography is a type of optical spectroscopy that uses light to measure the absorbance or emission intensity of a sample. The principle behind spectrophotometry involves measuring the change in absorbance as a function of wavelength, which can be used to determine the concentration of a substance at different wavelengths. Spectrophotometric analysis is commonly used in various fields such as medicine, biology, environmental science, and materials science to study and characterize biological systems.