Definition: SpMV stands for Single-Molecule Visual Microscopy. It is a technique used in biotechnology, specifically in the field of molecular biology, where cells are analyzed using light-emitting microscopes (LEMSs) to visualize their structure and function within living organisms. The term "spMV" refers to "single molecule visual microscopy," which highlights its ability to observe single molecules under specific conditions and magnification compared to traditional microscopic techniques like confocal microscopy or immunofluorescence microscopy, where