👉 The sole research, as requested, focuses on the development of a novel CRISPR-based gene editing tool designed to enhance the precision and efficiency of correcting genetic mutations in human embryos. This study introduces a modified Cas9 protein fused with a high-fidelity nuclease domain, significantly reducing off-target effects while maintaining robust on-target activity. By integrating machine learning algorithms to predict optimal guide RNA sequences, the research aims to streamline the editing process, making it more accessible for clinical applications in treating hereditary diseases such as cystic fibrosis and sickle cell anemia.
