👉 The replication fluid, often referred to as the replication vesicle or replication bubble, is a dynamic structure that forms during viral replication in host cells. It is a membrane-bound compartment where the viral genome replicates, often involving the hijacking of host cell machinery. The replication fluid contains viral RNA or DNA, along with essential viral proteins and enzymes, such as polymerases, which orchestrate the synthesis of new viral genetic material. This process occurs within the cytoplasm of the infected cell, facilitating the production of numerous copies of the virus, which are subsequently packaged into new virions for release and infection of additional cells.