👉 pMID (Programmed Mediated Interference) engineering is a computational approach that leverages machine learning to predict and enhance the performance of CRISPR-Cas9 gene editing systems. By analyzing extensive datasets of experimental outcomes, pMID models identify key sequence features and epigenetic markers that influence editing efficiency and specificity. These models can then be used to design optimized guide RNAs (gRNAs) with higher on-target activity and reduced off-target effects, streamlining the gene editing process and improving the reliability of genetic modifications. This technique represents a significant advancement in precision genome engineering, facilitating more efficient and accurate gene editing applications in research and therapeutic contexts.
