👉 Insertion engineering is a sophisticated technique used in the field of synthetic biology to precisely control and manipulate the behavior of biological systems by introducing specific genetic elements or modifications at targeted locations within a genome. This process involves the strategic insertion of DNA sequences, regulatory elements, or other genetic components into predefined sites to alter gene expression, introduce new functionalities, or reprogram cellular processes. Unlike random mutagenesis, insertion engineering employs tools such as CRISPR-Cas9, homologous recombination, or site-specific nucleases to achieve high precision and efficiency in genome editing. This method allows researchers to study gene functions, develop novel biological circuits, and engineer organisms for various applications, including biomanufacturing, medicine, and environmental remediation, by ensuring that genetic modifications occur exactly where intended.
