👉 Spectrophotometry is a method of measuring the concentration or absorbance of a molecule in an aqueous solution using light. It involves the use of a spectroscope, which consists of a light-sensitive glass prism that allows light to pass through it and be detected by a photomultiplier tube, which converts the light into electrical signals. The electrical signal is then displayed on a screen or computer monitor, allowing for easy analysis of the concentration of the molecule being measured.