👉 In biochemistry, a carboxylase is an enzyme that catalyzes the addition of carboxylic acids to substrates. Carboxylases are found in all eukaryotic cells and play important roles in a wide variety of metabolic processes. The term "carboxylase" has been used to describe a group of enzymes that have catalytic activity towards the formation of carboxylic acids, which can be either aldehyde or ketone derivatives. Carboxyl