👉 Fluorescence is a phenomenon in which light is emitted by a material or molecule, and this emission is detectable through the presence of an excited state. In the context of biological systems, fluorescence can be used to measure the activity or concentration of molecules, as well as for other applications such as imaging. In the field of chemistry, fluorescent molecules are those that emit light when they come into contact with a suitable chemical reagent. These reagents are often labeled with an ionizing agent (