👉 Colony fluid, also known as biofilm matrix, is a complex mixture of extracellular polymeric substances (EPS) that encases and protects microbial communities in various environments, including biofilms on surfaces. This matrix, primarily composed of polysaccharides, proteins, nucleic acids, and lipids, facilitates cell-to-cell communication and provides structural integrity, enabling the microorganisms within to adhere tightly to surfaces and resist environmental stresses such as desiccation, antibiotics, and immune responses. It plays a crucial role in the formation, stability, and function of microbial communities, influencing their interactions and survival in diverse habitats.